https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38723 Wed 19 Jan 2022 10:04:17 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:23091 Wed 11 Apr 2018 11:37:20 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:42559 Thu 25 Aug 2022 11:05:11 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:35167 Thu 11 Jul 2019 10:23:52 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:35166 Setaria viridis) has recently been proposed as a potential experimental model for the study of C₄ photosynthesis and is closely related to many economically important crop species of the Panicoideae subfamily of grasses, including Zea mays (maize), Sorghum bicolor (sorghum) and Sacchurum officinarum (sugarcane). Setaria viridis (Accession 10) possesses a number of key traits as an experimental model, namely; (i) a small sized, sequenced and well annotated genome; (ii) short stature and generation time; (iii) prolific seed production, and, (iv) is amendable to Agrobacterium tumefaciens-mediated transformation. There is currently however, a lack of reference gene expression information for Setaria viridis (S. viridis). We therefore aimed to identify a cohort of suitable S. viridis reference genes for accurate and reliable normalisation of S. viridis RT-qPCR expression data. Results: Eleven putative candidate reference genes were identified and examined across thirteen different S. viridis tissues. Of these, the geNorm and NormFinder analysis software identified SERINE/THERONINE-PROTEIN PHOSPHATASE 2A (PP2A), 5'-ADENYLYLSULFATE REDUCTASE 6 (ASPR6) and DUAL SPECIFICITY PHOSPHATASE (DUSP) as the most suitable combination of reference genes for the accurate and reliable normalisation of S. viridis RT-qPCR expression data. To demonstrate the suitability of the three selected reference genes, PP2A, ASPR6 and DUSP, were used to normalise the expression of CINNAMYL ALCOHOL DEHYDROGENASE (CAD) genes across the same tissues. Conclusions: This approach readily demonstrated the suitably of the three selected reference genes for the accurate and reliable normalisation of S. viridis RT-qPCR expression data. Further, the work reported here forms a highly useful platform for future gene expression quantification in S. viridis and can also be potentially directly translatable to other closely related and agronomically important C₄ crop species.]]> Mon 24 Jun 2019 16:46:06 AEST ]]>